Quantification of Biomolecules: Absorbance or Fluorescence? And the use of Fluorescence imaging technology that could help to develop Coronavirus testing kits
Fluorescence and UV-Vis absorbance are complementary measurement techniques for the quantification of biomolecules. Traditionally seen as separate, and sometimes competing methodologies, discuss the advantages of each as well as new systems that integrate multiple modes into single compact bench-top instruments.
The use of Fluorescence spectroscopy to sensitively detect RNA molecules, to help improve viral screening for viruses such as the Coronavirus COVID-19.
1- Basics of Absorbance Measurements
2- Basics of Fluorescence Quantification
3-Absorbance vs. Fluorescence
5-Quenching of Fluorescence
6-Live cell imaging of single RNA molecules with fluorogenic arrays
8-Use ACS format for references